Type Of Media：バイオ工学系専門誌
Publication/Magazine/Media：日経バイオテク
Director Ishii of National Institute of Health Science introduces methods for measuring particle size and purity of EVs formulations.
Summary:
The Innovation Center of NanoMedicine (iCONM) held a seminar titled "Want to Know More About Exosomes" on July 9, 2024. Akiko Ishii, head of the Biological Pharmaceuticals Division at the National Institute of Health Sciences, presented on "Exosomes: Future Pharmaceuticals," discussing methods for quality control when developing extracellular vesicles (EVs) as pharmaceuticals. She outlined five key areas for testing: particle size, composition, content, bioactivity, and single-particle analysis.
For measuring particle size, techniques such as nanoparticle tracking analysis and dynamic light scattering were highlighted, emphasizing the importance of understanding each method's characteristics. Regarding composition and purity, size-exclusion chromatography was recommended for evaluating impurities. In terms of content, particle number is preferred as an indicator, and the quantity of active ingredients can also be considered. Various methods for assessing bioactivity using cultured cells were introduced, with a focus on constructing evaluation systems based on mechanisms of action. Additionally, quality control methods for modified EVs were discussed. Other speakers included Associate Prof. Seo and Full Prof. Ichiki from University of Tokyo.
http://bio.nikkeibp.co.jp/atcl/news/p1/24/07/10/12135/?n_cid=nbpbto_mled_am
